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验证数据展示

  • WB analysis using 66787-1-Ig

    WB analysis using 66787-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66787-1-Ig (CRACR2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 66787-1-Ig

    WB analysis of Jurkat using 66787-1-Ig

    WB result of CRACR2A antibody (66787-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRACR2A transfected Jurkat cells.

  • IF Staining of HEK-293 using 66787-1-Ig

    IF Staining of HEK-293 using 66787-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66787-1-Ig (CRACR2A antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human colon using 66787-1-Ig

    IHC staining of human colon using 66787-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66787-1-Ig (CRACR2A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

CRACR2A Monoclonal antibody

CRACR2A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66787-1-Ig
CloneNo. 4C1F5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EFCAB4B, EF-hand calcium-binding domain-containing protein 4B, CRAC channel regulator 2A, Calcium release-activated channel regulator 2A, Calcium release-activated calcium channel regulator 2A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, Daudi cells, Ramos cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66787-1-Ig targets CRACR2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CRACR2A fusion protein Ag7525 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name EF-hand calcium binding domain 4B
Synonyms EFCAB4B, EF-hand calcium-binding domain-containing protein 4B, CRAC channel regulator 2A, Calcium release-activated channel regulator 2A, Calcium release-activated calcium channel regulator 2A
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC004524
Gene Symbol CRACR2A
Gene ID (NCBI) 84766
RRIDAB_2882132
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BSW2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRACR2A (also called CRAC regulator 2A, EFCAB4B) is a cytoplasmic Ca2+ sensor predominantly expressed in lymphocytes. It has two isoforms: a long isoform of CRACR2A-a round 80-90 kDa and a short isoform of CRACR2A-c around 46 kDa. CRACR2A-c is involved in the regulation of Ca2+-NFAT signaling. CRACR2A-a exists in a GTP- and Golgi membrane-bound form in resting T cells, while it is present in subsynaptic vesicles that translocated from the Golgi region to the immunological synapse after ligation of TCRs. This antibody recognizes both of CRACR2A-c and CRACR2A-a.

实验方案

Product Specific Protocols
WB protocol for CRACR2A antibody 66787-1-IgDownload protocol
IHC protocol for CRACR2A antibody 66787-1-IgDownload protocol
IF protocol for CRACR2A antibody 66787-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols