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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CRACR2A Monoclonal antibody

CRACR2A Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4C1F5

Cat no : CL594-66787

Print datasheet

Synonyms

CRAC channel regulator 2A, CRACR2A, DKFZp686G13246, EFCAB4B, FLJ33046, FLJ33805

验证数据展示

  • IF Staining of HEK-293 using CL594-66787

    IF Staining of HEK-293 using CL594-66787

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®594 CRACR2A antibody (CL594-66787, Clone: 4C1F5 ) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66787-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66787 targets CRACR2A in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen CRACR2A fusion protein Ag7525 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name EF-hand calcium binding domain 4B
Synonyms CRAC channel regulator 2A, CRACR2A, DKFZp686G13246, EFCAB4B, FLJ33046, FLJ33805
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC004524
Gene Symbol CRACR2A
Gene ID (NCBI) 84766
RRIDAB_2920038
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BSW2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRACR2A (also called CRAC regulator 2A, EFCAB4B) is a cytoplasmic Ca2+ sensor predominantly expressed in lymphocytes. It has two isoforms: a long isoform of CRACR2A-a round 80-90 kDa and a short isoform of CRACR2A-c around 46 kDa. CRACR2A-c is involved in the regulation of Ca2+-NFAT signaling. CRACR2A-a exists in a GTP- and Golgi membrane-bound form in resting T cells, while it is present in subsynaptic vesicles that translocated from the Golgi region to the immunological synapse after ligation of TCRs. This antibody recognizes both of CRACR2A-c and CRACR2A-a.

实验方案

Product Specific Protocols
IF protocol for CL594 CRACR2A antibody CL594-66787Download protocol
Standard Protocols
Click here to view our Standard Protocols