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验证数据展示

  • IF Staining of HEK-293 using CL488-66787

    IF Staining of HEK-293 using CL488-66787

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 CRACR2A antibody (CL488-66787, Clone: 4C1F5 ) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CRACR2A Monoclonal antibody

CRACR2A Monoclonal Antibody for IF/ICC
Cat No. CL488-66787

产品说明书

CloneNo. 4C1F5

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

IF/ICC

别名

CRAC channel regulator 2A, CRACR2A, DKFZp686G13246, EFCAB4B, FLJ33046, FLJ33805

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66787-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66787 targets CRACR2A in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 CRACR2A fusion protein Ag7525 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 EF-hand calcium binding domain 4B
别名 CRAC channel regulator 2A, CRACR2A, DKFZp686G13246, EFCAB4B, FLJ33046, FLJ33805
计算分子量 46 kDa
观测分子量 80-90 kDa
GenBank蛋白编号BC004524
基因名称 CRACR2A
Gene ID (NCBI) 84766
RRIDAB_2919370
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BSW2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRACR2A (also called CRAC regulator 2A, EFCAB4B) is a cytoplasmic Ca2+ sensor predominantly expressed in lymphocytes. It has two isoforms: a long isoform of CRACR2A-a round 80-90 kDa and a short isoform of CRACR2A-c around 46 kDa. CRACR2A-c is involved in the regulation of Ca2+-NFAT signaling. CRACR2A-a exists in a GTP- and Golgi membrane-bound form in resting T cells, while it is present in subsynaptic vesicles that translocated from the Golgi region to the immunological synapse after ligation of TCRs. This antibody recognizes both of CRACR2A-c and CRACR2A-a.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CRACR2A antibody CL488-66787Download protocol
Standard Protocols
Click here to view our Standard Protocols