• Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CREB1 Monoclonal antibody

CREB1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67927

产品说明书

CloneNo. 1E11C1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

FC (Intra)

Cyclic AMP-responsive element-binding protein 1, CREB-1, CREB 1, CREB, cAMP-responsive element-binding protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CREB1 Ready-To-Use IHC Kit. CREB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67927-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67927 targets CREB1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 CREB1 fusion protein Ag2852 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cAMP responsive element binding protein 1
别名 Cyclic AMP-responsive element-binding protein 1, CREB-1, CREB 1, CREB, cAMP-responsive element-binding protein 1
计算分子量 341 aa, 35 kDa
观测分子量 43-46 kDa
GenBank蛋白编号BC010636
基因名称 CREB1
Gene ID (NCBI) 1385
RRIDAB_2923827
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP16220
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CREB1 antibody CL488-67927Download protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...