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验证数据展示

  • WB analysis of HEK-293 using 67035-1-Ig

    WB analysis of HEK-293 using 67035-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

CRK Monoclonal antibody

CRK Monoclonal Antibody for WB, ELISA
Cat No. 67035-1-Ig

Print datasheet

CloneNo. 2B7E1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Biotin
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HSC-T6 cells, Jurkat cells, NIH/3T3 cells, RAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67035-1-Ig targets CRK in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen CRK fusion protein Ag28793 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name v-crk sarcoma virus CT10 oncogene homolog (avian)
Synonyms Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC008506
Gene Symbol CRK
Gene ID (NCBI) 1398
RRIDAB_2882350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP46108
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. CRK has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.

实验方案

Product Specific Protocols
WB protocol for CRK antibody 67035-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols