验证数据展示
经过测试的应用
| Positive ELISA detected in | Ag28793 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-67035 targets CRK in ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | ELISA Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 |
CatNo: Ag28793 Product name: Recombinant human CRK protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 41-304 aa of BC008506 Sequence: STSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLEFYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGNQEGSHPQPLGGPEPGPYAQPSVNTPLPNLQNGPIYARVIQKRVPNAYDKTALALEVGELVKVTKINVSGQWEGECNGKRGHFPFTHVRLLDQQNPDEDFS 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | v-crk sarcoma virus CT10 oncogene homolog (avian) |
| 别名 | Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk |
| 计算分子量 | 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC008506 |
| 基因名称 | CRK |
| Gene ID (NCBI) | 1398 |
| RRID | AB_3083813 |
| 偶联类型 | Biotin |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P46108 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. CRK has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.
