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Biotin-conjugated CRK Monoclonal antibody

CRK Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

ELISA

Conjugate

Biotin

CloneNo.

2B7E1

Cat no : Biotin-67035

Print datasheet

Synonyms

Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk

验证数据展示

  • ELISA experiment of Ag28793 using Biotin-67035

    ELISA experiment of Ag28793 using Biotin-67035

    Biotin-67035 tested by ELISA. Ag28793(CRK 41-304 aa)was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of Biotin-67035 (start dilution 1:500. Bound antibodies were detected with Streptavidin Poly-HRP(1:5000)followed by incubation with HRP Substrate,terminated with 2M H2SO4,then measuring the resulting absorbance at 450 nm.

经过测试的应用

Positive ELISA detected inAg28793

推荐稀释比

ApplicationDilution
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-67035 targets CRK in ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen CRK fusion protein Ag28793 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name v-crk sarcoma virus CT10 oncogene homolog (avian)
Synonyms Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC008506
Gene Symbol CRK
Gene ID (NCBI) 1398
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP46108
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. CRK has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.