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验证数据展示

  • FC experiment of SH-SY5Y using CL488-14686

    FC experiment of SH-SY5Y using CL488-14686

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Crmp2 Polyclonal Antibody (CL488-14686)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-14686

    IF Staining of HepG2 using CL488-14686

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CRMP2 antibody (CL488-14686) at dilution of 1:200.

CoraLite® Plus 488-conjugated CRMP2 Polyclonal antibody

CRMP2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-14686

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

DPYSL2, DRP-2, DRP2, Dihydropyrimidinase-related protein 2, DHPRP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy DPYSL2 Ready-To-Use IHC Kit. DPYSL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14686-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14686 targets CRMP2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 CRMP2 fusion protein Ag5985 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dihydropyrimidinase-like 2
别名 DPYSL2, DRP-2, DRP2, Dihydropyrimidinase-related protein 2, DHPRP2
计算分子量 62 kDa
观测分子量 62-66 kDa
GenBank蛋白编号BC056408
基因名称 CRMP2
Gene ID (NCBI) 1808
RRIDAB_3672599
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16555
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dihydropyrimidinase-like 2 (Dpysl2), a protein involved in neuronal differentiation and axonal guidance is found altered in various nervous system diseases (PMID:19524114). It is also named as CRMP2, ULIP2 and belongs to hydantoinase/dihydropyrimidinase subfamily. The phosphorylation of the particular portion carboxyl-terminal to the basic region of DPYSL2 may play an important role in regulating its activity, and may be involved in the formation of degenerating neurites in AD brain(PMID:10757975).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CRMP2 antibody CL488-14686Download protocol
Standard Protocols
Click here to view our Standard Protocols