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验证数据展示

  • WB analysis of Jurkat using 14504-1-AP (same clone as 14504-1-PBS)

    WB analysis of Jurkat using 14504-1-AP (same clone as 14504-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 14504-1-AP (same clone as 14504-1-PBS)

    IP experiment of HepG2 using 14504-1-AP (same clone as 14504-1-PBS)

    IP result of anti-CROP (IP:14504-1-AP, 4ug; Detection:14504-1-AP 1:500) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 14504-1-AP (same clone as 14504-1-PBS)

    IHC staining of human ovary cancer using 14504-1-AP (same clone as 14504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 14504-1-AP (CROP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 14504-1-AP (same clone as 14504-1-PBS)

    IF Staining of HepG2 using 14504-1-AP (same clone as 14504-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CROP antibody (14504-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14504-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CROP Polyclonal antibody, PBS Only

CROP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14504-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

CREAP-1, CREAP1, CREAP 1, CRE associated protein 1, Cisplatin resistance-associated-overexpressed protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

14504-1-PBS targets CROP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5961

Product name: Recombinant human CROP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-79 aa of BC056409

Sequence: MISAAQLLDELMGRDRNLAPDEKRSNVRWDHESVCKYYLCGFCPAELFTNTRSDLDVFGRGDNISDVSKFLEDDKWMEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cisplatin resistance-associated overexpressed protein
别名 CREAP-1, CREAP1, CREAP 1, CRE associated protein 1, Cisplatin resistance-associated-overexpressed protein
计算分子量 51 kDa
观测分子量 55-58 kDa
GenBank蛋白编号BC056409
基因名称 CROP
Gene ID (NCBI) 51747
RRIDAB_2229967
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95232
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CROP, a putative SR protein, contain cystine/histidine motifs and leucine zipper-like repeats in the N-terminal half and consists mostly of charged and polar amino acids in the C-terminal half. CROP has a high expression in heart, brain, pancreas, thymus, and weak in lung, live and kidney. As the human homologue of yeast Luc7p, CROP is supported to be participated in 5'-splice site recognize and is essential for vegetative growth. This is a rabbit polyclonal antibody raised against part of N-terminus of CROP of human origin, and can recognize a ~58kDa band of CROP.

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