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验证数据展示

  • IF Staining of HeLa using CL488-68276

    IF Staining of HeLa using CL488-68276

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 CRTAC1 antibody (CL488-68276, Clone: 2A11E2 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated CRTAC1 Monoclonal antibody

CRTAC1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68276

产品说明书

CloneNo. 2A11E2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

IF/ICC

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68276-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68276 targets CRTAC1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 CRTAC1 fusion protein Ag32813 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cartilage acidic protein 1
别名
计算分子量 661 aa, 71 kDa
观测分子量 71 kDa
GenBank蛋白编号BC034245
基因名称 CRTAC1
Gene ID (NCBI) 55118
RRIDAB_3672987
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NQ79
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRTAC1 (cartilage acidic protein 1) is also named as ASPIC1 and CEP68. CRTAC1 is an extracellular matrix protein of human chondrogenic tissue. CRTAC1 promotes cell proliferation, migration and extracellular matrix regeneration and remodeling in primary human fibroblasts (PMID:32084494). CRTAC1 expression is decreased in tissue samples of bladder urothelial carcinoma (PMID:34818994). CRTAC1 overexpression promotes the pyroptosis of human lens epithelial cells by facilitating the reactive oxygen species (ROS) production in the formation of cataract (PMID:32838966). CRTAC1 was the most compelling and robust biomarker for osteoarthritis severity and progression (PMID:35924962).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CRTAC1 antibody CL488-68276Download protocol
Standard Protocols
Click here to view our Standard Protocols