CoraLite® Plus 488-conjugated CRTAC1 Monoclonal antibody
CRTAC1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, pig, rabbit
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2A11E2
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 68276-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68276 targets CRTAC1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Immunogen | CRTAC1 fusion protein Ag32813 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cartilage acidic protein 1 |
| Synonyms | |
| Calculated Molecular Weight | 661 aa, 71 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC034245 |
| Gene Symbol | CRTAC1 |
| Gene ID (NCBI) | 55118 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NQ79 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRTAC1 (cartilage acidic protein 1) is also named as ASPIC1 and CEP68. CRTAC1 is an extracellular matrix protein of human chondrogenic tissue. CRTAC1 promotes cell proliferation, migration and extracellular matrix regeneration and remodeling in primary human fibroblasts (PMID:32084494). CRTAC1 expression is decreased in tissue samples of bladder urothelial carcinoma (PMID:34818994). CRTAC1 overexpression promotes the pyroptosis of human lens epithelial cells by facilitating the reactive oxygen species (ROS) production in the formation of cataract (PMID:32838966). CRTAC1 was the most compelling and robust biomarker for osteoarthritis severity and progression (PMID:35924962).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CRTAC1 antibody CL488-68276 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |