验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
产品信息
13319-1-AP targets CSDE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | CSDE1 fusion protein Ag4173 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cold shock domain containing E1, RNA-binding |
| 别名 | UNR, NRU, N-ras upstream gene protein, KIAA0885, Cold shock domain-containing protein E1 |
| 计算分子量 | 798 aa, 89 kDa |
| 观测分子量 | 89 kDa |
| GenBank蛋白编号 | BC032446 |
| 基因名称 | CSDE1 |
| Gene ID (NCBI) | 7812 |
| RRID | AB_2084902 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75534 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSDE1 (Cold shock domain-containing protein E1), also known as UNR (upstream of N-ras), is an RNA-binding protein involved in the regulation of messenger RNA stability and internal initiation of translation. It is required for internal initiation of translation of human rhinovirus RNA. UNR may be involved in translationally coupled mRNA turnover. Reduced expression of UNR impairs proliferation and differentiation of erythroid blasts. Recent study found that UNR acts as a positive or negative regulator of cell death, depending on the cell type, suggesting manipulation of UNR level may constitute a specific approach to sensitize cancer cells to anticancer treatments.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CSDE1 antibody 13319-1-AP | Download protocol |
| IHC protocol for CSDE1 antibody 13319-1-AP | Download protocol |
| IF protocol for CSDE1 antibody 13319-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Protoc Global analysis of RNA-binding protein dynamics by comparative and enhanced RNA interactome capture. | ||
Nat Commun Discovery of RNA-binding proteins and characterization of their dynamic responses by enhanced RNA interactome capture. | ||
Blood Ribosomal deficiencies in Diamond-Blackfan anemia impair translation of transcripts essential for differentiation of murine and human erythroblasts.
| ||
Viruses Upstream of N-Ras (Unr/CSDE1) Interacts with NCp7 and Gag, Modulating HIV-1 IRES-Mediated Translation Initiation
| ||
Nat Commun Systematic analysis of RNA-binding proteins identifies targetable therapeutic vulnerabilities in osteosarcoma | ||
Cell Rep The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network |