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  • Featured Product
  • KD/KO Validated

CSDE1 Polyclonal antibody

CSDE1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13319-1-AP

Print datasheet

Synonyms

UNR, NRU, N-ras upstream gene protein, KIAA0885, Cold shock domain-containing protein E1

验证数据展示

  • WB analysis using 13319-1-AP

    WB analysis using 13319-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13319-1-AP (CSDE1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 13319-1-AP

    IHC staining of human liver cancer using 13319-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13319-1-AP (CSDE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CSDE1 antibody included
  • IF Staining of HeLa using 13319-1-AP

    IF Staining of HeLa using 13319-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CSDE1 antibody (13319-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • WB analysis of A431 using 13319-1-AP

    WB analysis of A431 using 13319-1-AP

    WB result of CSDE1 antibody (13319-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSDE1 transfected A431 cells.

经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CSDE1 Ready-To-Use IHC Kit. CSDE1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13319-1-AP targets CSDE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen CSDE1 fusion protein Ag4173 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cold shock domain containing E1, RNA-binding
Synonyms UNR, NRU, N-ras upstream gene protein, KIAA0885, Cold shock domain-containing protein E1
Calculated Molecular Weight 798 aa, 89 kDa
Observed Molecular Weight 89 kDa
GenBank Accession NumberBC032446
Gene Symbol CSDE1
Gene ID (NCBI) 7812
RRIDAB_2084902
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75534
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSDE1 (Cold shock domain-containing protein E1), also known as UNR (upstream of N-ras), is an RNA-binding protein involved in the regulation of messenger RNA stability and internal initiation of translation. It is required for internal initiation of translation of human rhinovirus RNA. UNR may be involved in translationally coupled mRNA turnover. Reduced expression of UNR impairs proliferation and differentiation of erythroid blasts. Recent study found that UNR acts as a positive or negative regulator of cell death, depending on the cell type, suggesting manipulation of UNR level may constitute a specific approach to sensitize cancer cells to anticancer treatments.

实验方案

Product Specific Protocols
WB protocol for CSDE1 antibody 13319-1-APDownload protocol
IHC protocol for CSDE1 antibody 13319-1-APDownload protocol
IF protocol for CSDE1 antibody 13319-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Protoc

Global analysis of RNA-binding protein dynamics by comparative and enhanced RNA interactome capture.

Authors - Joel I Perez-Perri
View Article
humanWB

Nat Commun

Discovery of RNA-binding proteins and characterization of their dynamic responses by enhanced RNA interactome capture.

Authors - Joel I Perez-Perri
View Article
human,mouseWB

Blood

Ribosomal deficiencies in Diamond-Blackfan anemia impair translation of transcripts essential for differentiation of murine and human erythroblasts.

Authors - Horos Rastislav R
  • KD Validated
View Article
humanWB

Viruses

Upstream of N-Ras (Unr/CSDE1) Interacts with NCp7 and Gag, Modulating HIV-1 IRES-Mediated Translation Initiation

Authors - Nedal Taha
  • KD Validated
View Article
humanWB

Nat Commun

Systematic analysis of RNA-binding proteins identifies targetable therapeutic vulnerabilities in osteosarcoma

Authors - Yang Zhou
View Article

Cell Rep

The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network

Authors - Zhiying Yao
View Article