验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-13319 targets CSDE1 in IF/ICC applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | CSDE1 fusion protein Ag4173 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cold shock domain containing E1, RNA-binding |
| Synonyms | CSDE1, D1S155E, DKFZp779B0247, DKFZp779J1455, FLJ26882, KIAA0885, N ras upstream gene protein, NRU, Protein UNR, RP5 1000E10.3, UNR |
| Calculated Molecular Weight | 798 aa, 89 kDa |
| Observed Molecular Weight | 89 kDa |
| GenBank Accession Number | BC032446 |
| Gene Symbol | CSDE1 |
| Gene ID (NCBI) | 7812 |
| RRID | AB_3672561 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75534 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSDE1 (Cold shock domain-containing protein E1), also known as UNR (upstream of N-ras), is an RNA-binding protein involved in the regulation of messenger RNA stability and internal initiation of translation. It is required for internal initiation of translation of human rhinovirus RNA. UNR may be involved in translationally coupled mRNA turnover. Reduced expression of UNR impairs proliferation and differentiation of erythroid blasts. Recent study found that UNR acts as a positive or negative regulator of cell death, depending on the cell type, suggesting manipulation of UNR level may constitute a specific approach to sensitize cancer cells to anticancer treatments.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CSDE1 antibody CL488-13319 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |