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CoraLite® Plus 488-conjugated CSK Polyclonal antibody

CSK Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17720

Print datasheet

Synonyms

C Src kinase, c src tyrosine kinase, CSK, Protein tyrosine kinase CYL, Tyrosine protein kinase CSK

验证数据展示

  • IF Staining of A431 using CL488-17720

    IF Staining of A431 using CL488-17720

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 CSK antibody (CL488-17720) at dilution of 1:200.

  • FC experiment of A431 using CL488-17720

    FC experiment of A431 using CL488-17720

    1x10^6 A431 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 CSK Polyclonal Antibody (CL488-17720)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CSK Ready-To-Use IHC Kit. CSK primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17720-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17720 targets CSK in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, rat
Immunogen CSK fusion protein Ag12103 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name c-src tyrosine kinase
Synonyms C Src kinase, c src tyrosine kinase, CSK, Protein tyrosine kinase CYL, Tyrosine protein kinase CSK
Calculated Molecular Weight 450 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC106073
Gene Symbol CSK
Gene ID (NCBI) 1445
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP41240
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CSK antibody CL488-17720Download protocol
FC protocol for CL Plus 488 CSK antibody CL488-17720Download protocol
Standard Protocols
Click here to view our Standard Protocols