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验证数据展示

  • IF Staining of A431 using CL488-66115

    IF Staining of A431 using CL488-66115

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite® Plus 488-conjugated CSK antibody (CL488-66115, Clone: 3B12C5 ) at dilution of 1:100.

CoraLite® Plus 488-conjugated CSK Monoclonal antibody

CSK Monoclonal Antibody for IF/ICC
Cat No. CL488-66115

产品说明书

CloneNo. 3B12C5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

IF/ICC

别名

C Src kinase, c src tyrosine kinase, CSK, Protein tyrosine kinase CYL, Tyrosine protein kinase CSK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CSK Ready-To-Use IHC Kit. CSK primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66115-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66115 targets CSK in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 CSK fusion protein Ag12103 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 c-src tyrosine kinase
别名 C Src kinase, c src tyrosine kinase, CSK, Protein tyrosine kinase CYL, Tyrosine protein kinase CSK
计算分子量 450 aa, 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC106073
基因名称 CSK
Gene ID (NCBI) 1445
RRIDAB_2883262
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP41240
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CSK antibody CL488-66115Download protocol
Standard Protocols
Click here to view our Standard Protocols