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Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody, PBS Only

CTLA-4/CD152 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241100B8

Cat no : 98147-1-PBS

Print datasheet

Synonyms

Ctla4, Ly 56, Ctla 4, Cd152, 241100B8

验证数据展示

  • FC experiment of mouse splenocytes using 98147-1-RR (same clone as 98147-1-PBS)

    FC experiment of mouse splenocytes using 98147-1-RR (same clone as 98147-1-PBS)

    1x10^6 untreated or Con A treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody (98147-1-RR, Clone: 241100B8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were not fixed. This data was developed using the same antibody clone with 98147-1-PBS in a different storage buffer formulation.

  • FC experiment of mouse splenocytes using 98147-1-RR (same clone as 98147-1-PBS)

    FC experiment of mouse splenocytes using 98147-1-RR (same clone as 98147-1-PBS)

    1x10^6 Con A treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody (98147-1-RR, Clone: 241100B8)(red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody (98147-1-RR, Clone: 241100B8) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98147-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98147-1-RR

    Affinity and Kinetic Characterization of 98147-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98147-1-RR against Mouse CTLA-4/CD152 were performed. The affinity constant is 0.580 nM.

产品信息

98147-1-PBS targets CTLA-4/CD152 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytotoxic T-lymphocyte-associated protein 4
Synonyms Ctla4, Ly 56, Ctla 4, Cd152, 241100B8
Calculated Molecular Weight25 kDa
GenBank Accession NumberNM_009843.4
Gene Symbol CTLA-4
Gene ID (NCBI) 12477
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP09793
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).