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CoraLite® Plus 405 Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibody

CTLA-4/CD152 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

240793E8

Cat no : CL405-98078

Print datasheet

Synonyms

CTLA4, Cytotoxic T-lymphocyte protein 4, CTLA 4, CELIAC3, CD152

验证数据展示

  • FC experiment of human PBMCs using CL405-98078

    FC experiment of human PBMCs using CL405-98078

    1x10^6 unstimulated or PHA stimulated human PBMCs were surface stained with APC Anti-Human CD3 and 5 ul CoraLite® Plus 405 Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibody (CL405-98078, Clone:240793E8). Cells were not fixed.

  • FC experiment of human PBMCs using CL405-98078

    FC experiment of human PBMCs using CL405-98078

    1x10^6 PHA stimulated human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibody (CL405-98078, Clone:240793E8) (red), or CoraLite® Plus 405 Rabbit IgG Isotype Control Recombinant Antibody (CL405-98136, Clone: 240953C9)(blue). 1x10^6 unstimulated human PBMCs were stained with 5 ul CoraLite® Plus 405 Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibody (CL405-98078, Clone:240793E8) (black). Cells were not fixed.

经过测试的应用

Positive FC detected inPHA treated human PBMCs
For other applications, we recommend the unconjugated version of this antibody, 98078-1-PBS

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL405-98078 targets CTLA-4/CD152 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytotoxic T-lymphocyte-associated protein 4
Synonyms CTLA4, Cytotoxic T-lymphocyte protein 4, CTLA 4, CELIAC3, CD152
Calculated Molecular Weight 223 aa, 25 kDa
GenBank Accession NumberBC070162
Gene Symbol CTLA4
Gene ID (NCBI) 1493
Conjugate CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission Maxima Wavelengths399 nm / 422 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16410
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses.

实验方案

Product Specific Protocols
FC protocol for CL Plus 405 CTLA-4/CD152 antibody CL405-98078Download protocol
Standard Protocols
Click here to view our Standard Protocols