验证数据展示
经过测试的应用
| Positive FC detected in | PHA treated human PBMCs |
For other applications, we recommend the unconjugated version of this antibody, 98078-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL405-98078 targets CTLA-4/CD152 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | cytotoxic T-lymphocyte-associated protein 4 |
| 别名 | CTLA4, Cytotoxic T-lymphocyte protein 4, CTLA 4, CELIAC3, CD152 |
| 计算分子量 | 223 aa, 25 kDa |
| GenBank蛋白编号 | BC070162 |
| 基因名称 | CTLA4 |
| Gene ID (NCBI) | 1493 |
| 偶联类型 | CoraLite® Plus 405 Fluorescent Dye |
| 最大激发/发射波长 | 399 nm / 422 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P16410 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 405 CTLA-4/CD152 antibody CL405-98078 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |