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CUL1 Recombinant antibody, PBS Only

CUL1 Uni-rAbTM Recombinant Antibody for Indirect ELISA, IP, WB

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

Indirect ELISA, IP, WB

Conjugate

Unconjugated

CloneNo.

5K17

Cat no : 82474-1-PBS

Print datasheet

Synonyms

Cullin-1, Cullin1, CUL-1, 5K17

验证数据展示

  • WB analysis using 82474-1-RR (same clone as 82474-1-PBS)

    WB analysis using 82474-1-RR (same clone as 82474-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82474-1-RR (CUL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82474-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 82474-1-RR (same clone as 82474-1-PBS)

    WB analysis of A549 using 82474-1-RR (same clone as 82474-1-PBS)

    WB result of CUL1 antibody (82474-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL1 transfected A549 cells. This data was developed using the same antibody clone with 82474-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 82474-1-RR (same clone as 82474-1-PBS)

    IP experiment of HeLa using 82474-1-RR (same clone as 82474-1-PBS)

    IP result of anti-CUL1 (IP:82474-1-RR, 4ug; Detection:82474-1-RR 1:1000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82474-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82474-1-RR

    Affinity and Kinetic Characterization of 82474-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82474-1-RR against Human CUL1 were performed. The affinity constant is 21.7 nM.

Planning an IHC experiment? We recommend our IHCeasy CUL1 Ready-To-Use IHC Kit. CUL1 primary antibody included.

产品信息

82474-1-PBS targets CUL1 in Indirect ELISA, IP, WB applications and shows reactivity with Human, rat, mouse samples.

Tested Applications Indirect ELISA, IP, WB Application Description
Tested Reactivity Human, rat, mouse
Immunogen CUL1 fusion protein Ag3585 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cullin 1
Synonyms Cullin-1, Cullin1, CUL-1, 5K17
Calculated Molecular Weight 90 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC034318
Gene Symbol CUL1
Gene ID (NCBI) 8454
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13616
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.