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CUL1 Recombinant antibody

CUL1 Uni-rAbTM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

5K17

Cat no : 82474-1-RR

Print datasheet

Synonyms

Cullin-1, Cullin1, CUL-1, 5K17

验证数据展示

  • WB analysis using 82474-1-RR

    WB analysis using 82474-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82474-1-RR (CUL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 82474-1-RR

    WB analysis of A549 using 82474-1-RR

    WB result of CUL1 antibody (82474-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL1 transfected A549 cells.

  • IP experiment of HeLa using 82474-1-RR

    IP experiment of HeLa using 82474-1-RR

    IP result of anti-CUL1 (IP:82474-1-RR, 4ug; Detection:82474-1-RR 1:1000) with HeLa cells lysate 1280 ug.

  • Affinity and Kinetic Characterization of 82474-1-RR

    Affinity and Kinetic Characterization of 82474-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82474-1-RR against Human CUL1 were performed. The affinity constant is 21.7 nM.

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells, MCF-7 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CUL1 Ready-To-Use IHC Kit. CUL1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:17000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82474-1-RR targets CUL1 in WB, IP, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity Human, rat, mouse
Immunogen CUL1 fusion protein Ag3585 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cullin 1
Synonyms Cullin-1, Cullin1, CUL-1, 5K17
Calculated Molecular Weight 90 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC034318
Gene Symbol CUL1
Gene ID (NCBI) 8454
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13616
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

实验方案

Product Specific Protocols
WB protocol for CUL1 antibody 82474-1-RRDownload protocol
IP protocol for CUL1 antibody 82474-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols