CoraLite® Plus 488-conjugated CUL1 Recombinant antibody
CUL1 Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
Human, rat, mouse
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5K17
验证数据展示
经过测试的应用
| Positive IF detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy CUL1 Ready-To-Use IHC Kit. CUL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82474-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82474 targets CUL1 in IF applications and shows reactivity with Human, rat, mouse samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, rat, mouse |
| Immunogen | CUL1 fusion protein Ag3585 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | cullin 1 |
| Synonyms | CUL 1, CUL1, cullin 1 |
| Calculated Molecular Weight | 90 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC034318 |
| Gene Symbol | CUL1 |
| Gene ID (NCBI) | 8454 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13616 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CUL1 antibody CL488-82474 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |