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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CUL1 Recombinant antibody

CUL1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5K17

Cat no : CL488-82474

Print datasheet

Synonyms

Cullin-1, Cullin1, CUL-1, 5K17

验证数据展示

  • IF Staining of HEK-293 using CL488-82474

    IF Staining of HEK-293 using CL488-82474

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 CUL1 antibody (CL488-82474, Clone: 5K17 ) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-82474

    FC experiment of HeLa using CL488-82474

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated CUL1 Recombinant antibody (CL488-82474, Clone:5K17)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CUL1 Ready-To-Use IHC Kit. CUL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82474-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82474 targets CUL1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen CUL1 fusion protein Ag3585 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cullin 1
Synonyms Cullin-1, Cullin1, CUL-1, 5K17
Calculated Molecular Weight 90 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC034318
Gene Symbol CUL1
Gene ID (NCBI) 8454
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13616
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CUL1 antibody CL488-82474Download protocol
Standard Protocols
Click here to view our Standard Protocols