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  • KD/KO Validated

CUL4B Polyclonal antibody

CUL4B Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IF/ICC, IP, ELISA and More (4)

Conjugate

Unconjugated

Cat no : 12916-1-AP

Print datasheet

Synonyms

KIAA0695, Cullin-4B, cullin 4B



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CUL4B Ready-To-Use IHC Kit. CUL4B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12916-1-AP targets CUL4B in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, RIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, canine
Immunogen CUL4B fusion protein Ag3563 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cullin 4B
Synonyms KIAA0695, Cullin-4B, cullin 4B
Calculated Molecular Weight 913 aa, 104 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC036216
Gene Symbol CUL4B
Gene ID (NCBI) 8450
RRIDAB_2086699
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13620
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).

实验方案

Product Specific Protocols
WB protocol for CUL4B antibody 12916-1-APDownload protocol
IF protocol for CUL4B antibody 12916-1-APDownload protocol
IP protocol for CUL4B antibody 12916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Targeted protein degradation via intramolecular bivalent glues

Authors - Oliver Hsia
  • KD Validated
humanCoIP,WB

Cell

FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells.

Authors - Jia Z Shen
  • KD Validated
humanWB

Cell

Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.

Authors - Xiulei Mo
humanIHC,IF

Science

CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.

Authors - Chao Yu
humanWB

Nat Med

Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity.

Authors - Ruth Eichner
  • KD Validated
humanWB

Science

Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.

Authors - Ting Han