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CUL4B Polyclonal antibody

CUL4B Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 12916-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (2)

应用

WB, IF/ICC, IP, ELISA and More (4)

KIAA0695, Cullin-4B, cullin 4B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CUL4B Ready-To-Use IHC Kit. CUL4B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12916-1-AP targets CUL4B in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, RIP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, canine
免疫原 CUL4B fusion protein Ag3563 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cullin 4B
别名 KIAA0695, Cullin-4B, cullin 4B
计算分子量 913 aa, 104 kDa
观测分子量 102 kDa
GenBank蛋白编号BC036216
基因名称 CUL4B
Gene ID (NCBI) 8450
RRIDAB_2086699
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13620
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329). This antibody may detect both CUL4A (87 kDa) and CUL4B(104 kDa).

实验方案

Product Specific Protocols
WB protocol for CUL4B antibody 12916-1-APDownload protocol
IF protocol for CUL4B antibody 12916-1-APDownload protocol
IP protocol for CUL4B antibody 12916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Targeted protein degradation via intramolecular bivalent glues

Authors - Oliver Hsia
  • KD Validated
humanCoIP,WB

Cell

FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells.

Authors - Jia Z Shen
  • KD Validated
humanWB

Cell

Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.

Authors - Xiulei Mo
humanIHC,IF

Science

CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.

Authors - Chao Yu
humanWB

Nat Med

Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity.

Authors - Ruth Eichner
  • KD Validated
humanWB

Science

Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.

Authors - Ting Han
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