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验证数据展示

  • FC experiment of HeLa using CL488-12916

    FC experiment of HeLa using CL488-12916

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CUL4B (CL488-12916) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CUL4B Polyclonal antibody

CUL4B Polyclonal Antibody for FC (Intra)
Cat No. CL488-12916

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Synonyms

CUL 4B, CUL4B, cullin 4B, DKFZp686F1470, KIAA0695, MRXHF2, MRXSC, SFM2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CUL4B Ready-To-Use IHC Kit. CUL4B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12916-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12916 targets CUL4B in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen CUL4B fusion protein Ag3563 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cullin 4B
Synonyms CUL 4B, CUL4B, cullin 4B, DKFZp686F1470, KIAA0695, MRXHF2, MRXSC, SFM2
Calculated Molecular Weight 913 aa, 104 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC036216
Gene Symbol CUL4B
Gene ID (NCBI) 8450
RRIDAB_2919062
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13620
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CUL4B antibody CL488-12916Download protocol
Standard Protocols
Click here to view our Standard Protocols