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CoraLite®594-conjugated CUL4B Polyclonal antibody
CUL4B Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | HeLa cells |
Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CUL4B Ready-To-Use IHC Kit. CUL4B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12916-1-AP
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-12916 targets CUL4B in FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human, mouse |
Immunogen | CUL4B fusion protein Ag3563 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cullin 4B |
Synonyms | CUL 4B, CUL4B, cullin 4B, DKFZp686F1470, KIAA0695, MRXHF2, MRXSC, SFM2 |
Calculated Molecular Weight | 913 aa, 104 kDa |
Observed Molecular Weight | 102 kDa |
GenBank Accession Number | BC036216 |
Gene Symbol | CUL4B |
Gene ID (NCBI) | 8450 |
RRID | AB_2919790 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13620 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).
实验方案
Product Specific Protocols | |
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FC protocol for CL594 CUL4B antibody CL594-12916 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |