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验证数据展示

  • IF Staining of HeLa using CL594-12916

    IF Staining of HeLa using CL594-12916

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 CUL4B antibody (CL594-12916) at dilution of 1:200, CL488-phalloidin (green).

  • IF Staining of HepG2 using CL594-12916

    IF Staining of HepG2 using CL594-12916

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 CUL4B antibody (CL594-12916) at dilution of 1:200, CL488-phalloidin (green).

  • FC experiment of HeLa using CL594-12916

    FC experiment of HeLa using CL594-12916

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CUL4B (CL594-12916) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated CUL4B Polyclonal antibody

CUL4B Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-12916

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

cullin 4B, Cullin-4B, KIAA0695

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

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经过测试的应用

Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CUL4B Ready-To-Use IHC Kit. CUL4B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12916-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-12916 targets CUL4B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 CUL4B fusion protein Ag3563 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cullin 4B
别名 cullin 4B, Cullin-4B, KIAA0695
计算分子量 913 aa, 104 kDa
观测分子量 102 kDa
GenBank蛋白编号BC036216
基因名称 CUL4B
Gene ID (NCBI) 8450
RRIDAB_2919790
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13620
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).

实验方案

Product Specific Protocols
IF protocol for CL594 CUL4B antibody CL594-12916Download protocol
FC protocol for CL594 CUL4B antibody CL594-12916Download protocol
Standard Protocols
Click here to view our Standard Protocols