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CoraLite® Plus 488-conjugated CXCL12/SDF-1 Polyclonal antibody
CXCL12/SDF-1 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | Jurkat cells |
Positive FC detected in | Jurkat cells |
For other applications, we recommend the unconjugated version of this antibody, 17402-1-AP
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-17402 targets CXCL12/SDF-1 in FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human, mouse |
Immunogen | CXCL12/SDF-1 fusion protein Ag11379 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) |
Synonyms | C X C motif chemokine 12, CXCL12, CXCL12/SDF-1, hIRH, hSDF 1, IRH, PBSF, SCYB12, SDF 1, SDF 1a, SDF 1b, SDF1, SDF-1, SDF1A, SDF1B, Stromal cell derived factor 1, TLSF a, TLSF b, TPAR1 |
Calculated Molecular Weight | 89 aa, 10 kDa |
GenBank Accession Number | BC039893 |
Gene Symbol | CXCL12/SDF-1 |
Gene ID (NCBI) | 6387 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P48061 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
In adulthood, CXCL12 plays an important role in angiogenesis by recruiting endothelial progenitor cells (EPCs) from the bone marrow through a CXCR4 dependent mechanism (PMID: 17878755). It is this function of CXCL12 that makes it a very important factor in carcinogenesis and the neovascularisation linked to tumour progression (PMID: 16943240). CXCL12 also has a role in tumor metastasis where cancer cells that express the receptor CXCR4 are attracted to metastasis target tissues that release the ligand, CXCL12 (PMID: 11242036 ). In breast cancer, however, increased expression of CXCL12 determines a reduced risk of distant metastasis (PMID: 19646861; 17724466 ). This antibody can detect all the isoforms of CXCL12/SDF1.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 CXCL12/SDF-1 antibody CL488-17402 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |