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验证数据展示

  • IHC staining of mouse spleen using Biotin-11073

    IHC staining of mouse spleen using Biotin-11073

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using Biotin-11073 (CXCR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CXCR4 antibody included
  • IHC staining of mouse spleen using Biotin-11073

    IHC staining of mouse spleen using Biotin-11073

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using Biotin-11073 (CXCR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CXCR4 antibody included
  • Featured Product
  • KD/KO Validated

Biotin-conjugated CXCR4 Polyclonal antibody

CXCR4 Polyclonal Antibody for IHC
Cat No. Biotin-11073

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Synonyms

CD184, CXC R4, CXCR 4, CXCR4, CXCR4B, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP3, LCR1, LESTR, SDF 1 receptor, WHIM

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  {{ptg:cur_Conjugation}}
Size: 

-/ -

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经过测试的应用

Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CXCR4 Ready-To-Use IHC Kit. CXCR4 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-11073 targets CXCR4 in IHC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC Application Description
Tested Reactivity human, mouse, rat
Immunogen CXCR4 fusion protein Ag1528 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chemokine (C-X-C motif) receptor 4
Synonyms CD184, CXC R4, CXCR 4, CXCR4, CXCR4B, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP3, LCR1, LESTR, SDF 1 receptor, WHIM
Calculated Molecular Weight 352 aa, 40 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC020968
Gene Symbol CXCR4
Gene ID (NCBI) 7852
RRIDAB_2918935
Conjugate Biotin
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61073
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some researches, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).

实验方案

Product Specific Protocols
IHC protocol for Biotin CXCR4 antibody Biotin-11073Download protocol
Standard Protocols
Click here to view our Standard Protocols