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验证数据展示

  • IHC staining of mouse spleen using Biotin-11073

    IHC staining of mouse spleen using Biotin-11073

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using Biotin-11073 (CXCR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CXCR4 antibody included
  • IHC staining of mouse spleen using Biotin-11073

    IHC staining of mouse spleen using Biotin-11073

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using Biotin-11073 (CXCR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CXCR4 antibody included
  • Featured Product
  • KD/KO验证

Biotin-conjugated CXCR4 Polyclonal antibody

CXCR4 Polyclonal Antibody for IHC
Cat No. Biotin-11073

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC

别名

CD184, CXC R4, CXCR 4, CXCR4, CXCR4B, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP3, LCR1, LESTR, SDF 1 receptor, WHIM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CXCR4 Ready-To-Use IHC Kit. CXCR4 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-11073 targets CXCR4 in IHC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC Application Description
经测试反应性 human, mouse, rat
免疫原 CXCR4 fusion protein Ag1528 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-X-C motif) receptor 4
别名 CD184, CXC R4, CXCR 4, CXCR4, CXCR4B, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP3, LCR1, LESTR, SDF 1 receptor, WHIM
计算分子量 352 aa, 40 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC020968
基因名称 CXCR4
Gene ID (NCBI) 7852
RRIDAB_2918935
偶联类型 Biotin
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61073
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some researches, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).

实验方案

Product Specific Protocols
IHC protocol for Biotin CXCR4 antibody Biotin-11073Download protocol
Standard Protocols
Click here to view our Standard Protocols