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CXCR4 Polyclonal antibody

CXCR4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11073-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA and More (3)

CXCR-4, CXC-R4, CXCR 4, CXC R4, C-X-C chemokine receptor type 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
Biotin
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CXCR4 Ready-To-Use IHC Kit. CXCR4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11073-2-AP targets CXCR4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 CXCR4 fusion protein Ag1528 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-X-C motif) receptor 4
别名 CXCR-4, CXC-R4, CXCR 4, CXC R4, C-X-C chemokine receptor type 4
计算分子量 352 aa, 40 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC020968
基因名称 CXCR4
Gene ID (NCBI) 7852
RRIDAB_2091813
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61073
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some research, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).

实验方案

Product Specific Protocols
WB protocol for CXCR4 antibody 11073-2-APDownload protocol
IHC protocol for CXCR4 antibody 11073-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Authors - Xiao-Hui Wang
humanWB

Nat Commun

N6-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis.

Authors - Ri-Xin Chen
ratWB

Acta Biomater

Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.

Authors - Shanshan Ma
mouseWB

Theranostics

Bioengineering CXCR4-overexpressing cell membrane functionalized ROS-responsive nanotherapeutics for targeting cerebral ischemia-reperfusion injury.

Authors - Li Luo
ratWB,IHC,IF

Biomater Sci

The effects of Sr-incorporated micro/nano rough titanium surface on rBMSC migration and osteogenic differentiation for rapid osteointegration

Authors - Chuan Zhou
mouseWB

Brain Behav Immun

Cerebral ischemia increases bone marrow CD4(+)CD25(+)FoxP3(+) regulatory T cells in mice via signals from sympathetic nervous system.

Authors - Jianping Wang
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