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Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody

CXCR4/CD184 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241735F8

Cat no : 98175-1-RR

Print datasheet

Synonyms

CXCR4, CXCR 4, CXC R4, C-X-C chemokine receptor type 4, CD184

验证数据展示

  • FC experiment of human PBMCs using 98175-1-RR

    FC experiment of human PBMCs using 98175-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody (98175-1-RR, Clone: 241735F8),or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98175-1-RR

    FC experiment of human PBMCs using 98175-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody (98175-1-RR, Clone:241735F8)(red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CXCR4 Ready-To-Use IHC Kit. CXCR4 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98175-1-RR targets CXCR4/CD184 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-X-C motif) receptor 4
Synonyms CXCR4, CXCR 4, CXC R4, C-X-C chemokine receptor type 4, CD184
Calculated Molecular Weight 352 aa, 40 kDa
GenBank Accession NumberBC020968
Gene Symbol CXCR4
Gene ID (NCBI) 7852
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP61073
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

C-X-C chemokine receptor type 4 (CXCR4, also known as CD184) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609).

实验方案

Product Specific Protocols
FC protocol for CXCR4/CD184 antibody 98175-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols