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验证数据展示

  • FC experiment of human PBMCs using PE-98175

    FC experiment of human PBMCs using PE-98175

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7), and 5 ul PE Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody (PE-98175, Clone: 241735F8) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9). Cells were not fixed.

  • FC experiment of human PBMCs using PE-98175

    FC experiment of human PBMCs using PE-98175

    1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody (PE-98175, Clone: 241735F8) (red), or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9) (blue). Cells were not fixed.

PE Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody

CXCR4/CD184 Recombinant Antibody for FC
Cat No. PE-98175

Print datasheet

CloneNo. 241735F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CXCR4, CXCR 4, CXC R4, C-X-C chemokine receptor type 4, CD184

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  PE
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CXCR4 Ready-To-Use IHC Kit. CXCR4 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-98175 targets CXCR4/CD184 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-X-C motif) receptor 4
Synonyms CXCR4, CXCR 4, CXC R4, C-X-C chemokine receptor type 4, CD184
Calculated Molecular Weight 352 aa, 40 kDa
GenBank Accession NumberBC020968
Gene Symbol CXCR4
Gene ID (NCBI) 7852
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61073
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

C-X-C chemokine receptor type 4 (CXCR4, also known as CD184) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609).

实验方案

Product Specific Protocols
FC protocol for PE CXCR4/CD184 antibody PE-98175Download protocol
Standard Protocols
Click here to view our Standard Protocols