• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human PBMCs using PE-98175

    FC experiment of human PBMCs using PE-98175

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7), and 5 ul PE Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody (PE-98175, Clone: 241735F8) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9). Cells were not fixed.

  • FC experiment of human PBMCs using PE-98175

    FC experiment of human PBMCs using PE-98175

    1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody (PE-98175, Clone: 241735F8) (red), or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9) (blue). Cells were not fixed.

PE Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody

CXCR4/CD184 Recombinant Antibody for FC
Cat No. PE-98175

产品说明书

CloneNo. 241735F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CXCR4, CXCR 4, CXC R4, C-X-C chemokine receptor type 4, CD184

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  PE
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CXCR4 Ready-To-Use IHC Kit. CXCR4 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-98175 targets CXCR4/CD184 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-X-C motif) receptor 4
别名 CXCR4, CXCR 4, CXC R4, C-X-C chemokine receptor type 4, CD184
计算分子量 352 aa, 40 kDa
GenBank蛋白编号BC020968
基因名称 CXCR4
Gene ID (NCBI) 7852
偶联类型 PE Fluorescent Dye
最大激发/发射波长496 nm, 565 nm / 578 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61073
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

C-X-C chemokine receptor type 4 (CXCR4, also known as CD184) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609).

实验方案

Product Specific Protocols
FC protocol for PE CXCR4/CD184 antibody PE-98175Download protocol
Standard Protocols
Click here to view our Standard Protocols