验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, U-87 MG cells, Jurkat cells, Raji cells, K-562 cells, THP-1 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue, human prostate cancer tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
60216-1-Ig targets CXCR7 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | CXCR7 fusion protein Ag14247 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | chemokine (C-X-C motif) receptor 7 |
| 别名 | ACKR3, CXC R7, C-X-C chemokine receptor type 7, CMKOR1, Chemokine orphan receptor 1 |
| 计算分子量 | 362 aa, 41 kDa |
| 观测分子量 | 50-60 kDa |
| GenBank蛋白编号 | BC036661 |
| 基因名称 | CXCR7 |
| Gene ID (NCBI) | 57007 |
| RRID | AB_11182925 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P25106 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as a coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CXCR7 antibody 60216-1-Ig | Download protocol |
| IHC protocol for CXCR7 antibody 60216-1-Ig | Download protocol |
| IF protocol for CXCR7 antibody 60216-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |