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验证数据展示

  • IF Staining of SW480 using CL488-60216

    IF Staining of SW480 using CL488-60216

    Immunofluorescent analysis of (-20°C Methanol) fixed SW480 cells using CoraLite® Plus 488 CXCR7 antibody (CL488-60216, Clone: 4C3D7 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated CXCR7 Monoclonal antibody

CXCR7 Monoclonal Antibody for IF/ICC
Cat No. CL488-60216

产品说明书

CloneNo. 4C3D7

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

IF/ICC

别名

ACKR3, Chemokine orphan receptor 1, CMKOR1, CXC R7, CXCR 7, CXCR7, G protein coupled receptor 159, GPR159, RDC 1, RDC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSW480 cells
For other applications, we recommend the unconjugated version of this antibody, 60216-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60216 targets CXCR7 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 CXCR7 fusion protein Ag14247 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 chemokine (C-X-C motif) receptor 7
别名 ACKR3, Chemokine orphan receptor 1, CMKOR1, CXC R7, CXCR 7, CXCR7, G protein coupled receptor 159, GPR159, RDC 1, RDC1
计算分子量 362 aa, 41 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC036661
基因名称 CXCR7
Gene ID (NCBI) 57007
RRIDAB_3672862
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25106
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CXCR7 antibody CL488-60216Download protocol
Standard Protocols
Click here to view our Standard Protocols