验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, human liver tissue, human kidney tissue, MCF-7 cells, mouse liver tissue |
Positive IHC detected in | human liver cancer tissue, human bowen disease Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
ChIP | See 1 publications below |
产品信息
12286-1-AP targets CYP4F11 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, ChIP |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | CYP4F11 fusion protein Ag2906 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cytochrome P450, family 4, subfamily F, polypeptide 11 |
Synonyms | EC:1.14.14.78, EC:1.14.14.1, Docosahexaenoic acid omega-hydroxylase, Cytochrome P450 4F11, CYPIVF11 |
Calculated Molecular Weight | 524 aa, 60 kDa |
Observed Molecular Weight | 57 kDa |
GenBank Accession Number | BC016853 |
Gene Symbol | CYP4F11 |
Gene ID (NCBI) | 57834 |
RRID | AB_2088407 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9HBI6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CYP4F11, also named as CYPIVF11, belongs to the cytochrome P450 family. It is the lone CYP4F/A enzyme that effectively oxidized 3-hydroxypalmitate. It is the predominant catalyst of this fatty acid oxidation reaction in human liver. CYP4F11 is one of the primary human epidermal keratinocytes. It is positively regulated by multiple signaling pathways in HaCaT keratinocytes, including RXR and JNK signaling pathways. (PMID:19812349)
实验方案
Product Specific Protocols | |
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WB protocol for CYP4F11 antibody 12286-1-AP | Download protocol |
IHC protocol for CYP4F11 antibody 12286-1-AP | Download protocol |
IF protocol for CYP4F11 antibody 12286-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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