验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, human liver tissue, human kidney tissue, MCF-7 cells, mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, human bowen disease Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| ChIP | See 1 publications below |
产品信息
12286-1-AP targets CYP4F11 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | CYP4F11 fusion protein Ag2906 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome P450, family 4, subfamily F, polypeptide 11 |
| 别名 | EC:1.14.14.78, EC:1.14.14.1, Docosahexaenoic acid omega-hydroxylase, Cytochrome P450 4F11, CYPIVF11 |
| 计算分子量 | 524 aa, 60 kDa |
| 观测分子量 | 57 kDa |
| GenBank蛋白编号 | BC016853 |
| 基因名称 | CYP4F11 |
| Gene ID (NCBI) | 57834 |
| RRID | AB_2088407 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HBI6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CYP4F11, also named as CYPIVF11, belongs to the cytochrome P450 family. It is the lone CYP4F/A enzyme that effectively oxidized 3-hydroxypalmitate. It is the predominant catalyst of this fatty acid oxidation reaction in human liver. CYP4F11 is one of the primary human epidermal keratinocytes. It is positively regulated by multiple signaling pathways in HaCaT keratinocytes, including RXR and JNK signaling pathways. (PMID:19812349)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP4F11 antibody 12286-1-AP | Download protocol |
| IHC protocol for CYP4F11 antibody 12286-1-AP | Download protocol |
| IF protocol for CYP4F11 antibody 12286-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
