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  • Featured Product
  • KD/KO Validated

CYP4F11 Polyclonal antibody

CYP4F11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12286-1-AP

Print datasheet

Synonyms

EC:1.14.14.78, EC:1.14.14.1, Docosahexaenoic acid omega-hydroxylase, Cytochrome P450 4F11, CYPIVF11

验证数据展示

  • WB analysis of MCF-7 using 12286-1-AP

    WB analysis of MCF-7 using 12286-1-AP

    WB result of CYP4F11 antibody (12286-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP4F11 transfected MCF-7 cells.

  • WB analysis using 12286-1-AP

    WB analysis using 12286-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12286-1-AP (CYP4F11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 12286-1-AP

    IHC staining of human liver cancer using 12286-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12286-1-AP (CYP4F11 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 12286-1-AP

    IHC staining of human liver cancer using 12286-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12286-1-AP (CYP4F11 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 12286-1-AP

    IF Staining of A549 using 12286-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CYP4F11 antibody (12286-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human bowen disease using 12286-1-AP

    IHC staining of human bowen disease using 12286-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12286-1-AP (CYP4F11 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, human liver tissue, human kidney tissue, MCF-7 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12286-1-AP targets CYP4F11 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, ChIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen CYP4F11 fusion protein Ag2906 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 4, subfamily F, polypeptide 11
Synonyms EC:1.14.14.78, EC:1.14.14.1, Docosahexaenoic acid omega-hydroxylase, Cytochrome P450 4F11, CYPIVF11
Calculated Molecular Weight 524 aa, 60 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC016853
Gene Symbol CYP4F11
Gene ID (NCBI) 57834
RRIDAB_2088407
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBI6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYP4F11, also named as CYPIVF11, belongs to the cytochrome P450 family. It is the lone CYP4F/A enzyme that effectively oxidized 3-hydroxypalmitate. It is the predominant catalyst of this fatty acid oxidation reaction in human liver. CYP4F11 is one of the primary human epidermal keratinocytes. It is positively regulated by multiple signaling pathways in HaCaT keratinocytes, including RXR and JNK signaling pathways. (PMID:19812349)

实验方案

Product Specific Protocols
WB protocol for CYP4F11 antibody 12286-1-APDownload protocol
IHC protocol for CYP4F11 antibody 12286-1-APDownload protocol
IF protocol for CYP4F11 antibody 12286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Drug Metab Dispos

Gene regulation of CYP4F11 in human keratinocyte HaCaT cells.

Authors - Wang Ying Y
View Article
humanChIP

Drug Metab Dispos

Regulation of cytochrome P450 4F11 by nuclear transcription factor-κB.

Authors - Bell Jordan C JC
View Article