验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67880-1-Ig targets Cyclophilin A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | Cyclophilin A fusion protein Ag1120 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | peptidylprolyl isomerase A (cyclophilin A) |
别名 | PPIA, EC:5.2.1.8, CYPH, CYPA, Cyclosporin A-binding protein |
计算分子量 | 18 kDa |
观测分子量 | 18 kDa |
GenBank蛋白编号 | BC005320 |
基因名称 | PPIA |
Gene ID (NCBI) | 5478 |
RRID | AB_2918637 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P62937 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclophilin A, also named as PPIA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.
实验方案
Product Specific Protocols | |
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WB protocol for Cyclophilin A antibody 67880-1-Ig | Download protocol |
IF protocol for Cyclophilin A antibody 67880-1-Ig | Download protocol |
FC protocol for Cyclophilin A antibody 67880-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |