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验证数据展示

  • IF Staining of HeLa using CL488-67880

    IF Staining of HeLa using CL488-67880

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cyclophilin A antibody (CL488-67880, Clone: 2B11B9 ) at dilution of 1:400.

  • FC experiment of HeLa using CL488-67880

    FC experiment of HeLa using CL488-67880

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Cyclophilin A (CL488-67880, Clone:2B11B9) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated Cyclophilin A Monoclonal antibody

Cyclophilin A Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67880

产品说明书

CloneNo. 2B11B9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

PPIA, EC:5.2.1.8, CYPH, CYPA, Cyclosporin A-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PPIA Ready-To-Use IHC Kit. PPIA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67880-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67880 targets Cyclophilin A in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 Cyclophilin A fusion protein Ag1120 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 peptidylprolyl isomerase A (cyclophilin A)
别名 PPIA, EC:5.2.1.8, CYPH, CYPA, Cyclosporin A-binding protein
计算分子量 18 kDa
观测分子量18 kDa
GenBank蛋白编号BC005320
基因名称 PPIA
Gene ID (NCBI) 5478
RRIDAB_2923813
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP62937
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclophilin A, also named as PPIA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cyclophilin A antibody CL488-67880Download protocol
Standard Protocols
Click here to view our Standard Protocols