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CoraLite® Plus 488-conjugated Calnexin Monoclonal antibody

Calnexin Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A2C6

Cat No : CL488-66903

Print datasheet

Synonyms

calnexin, CANX, CNX, FLJ26570, IP90, P90

验证数据展示

  • IF Staining of HepG2 using CL488-66903

    IF Staining of HepG2 using CL488-66903

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66903 (Calnexin antibody) at dilution of 1:100.

经过测试的应用

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Calnexin Ready-To-Use IHC Kit. Calnexin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66903-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66903 targets Calnexin in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen Calnexin fusion protein Ag25535 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calnexin
Synonyms calnexin, CANX, CNX, FLJ26570, IP90, P90
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC003552
Gene Symbol CANX
Gene ID (NCBI) 821
RRIDAB_2883391
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP27824
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Calnexin antibody CL488-66903Download protocol
Standard Protocols
Click here to view our Standard Protocols