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验证数据展示

  • WB analysis using CL594-66903

    WB analysis using CL594-66903

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-66903 (Calnexin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using CL594-66903

    IF Staining of HepG2 using CL594-66903

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 Calnexin antibody (CL594-66903, Clone: 2A2C6 ) at dilution of 1:200.

  • IF Staining of SKOV-3 using CL594-66903

    IF Staining of SKOV-3 using CL594-66903

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite®594 Calnexin antibody (CL594-66903, Clone: 2A2C6 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated Calnexin Monoclonal antibody

Calnexin Monoclonal Antibody for WB, IF/ICC
Cat No. CL594-66903

产品说明书

CloneNo. 2A2C6

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC

别名

calnexin, CANX, CNX, FLJ26570, IP90, P90

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inHepG2 cells, SKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy Calnexin Ready-To-Use IHC Kit. Calnexin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66903-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66903 targets Calnexin in WB, IF/ICC applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC Application Description
经测试反应性 Human
免疫原 Calnexin fusion protein Ag25535 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 calnexin
别名 calnexin, CANX, CNX, FLJ26570, IP90, P90
计算分子量 90 kDa
观测分子量 90 kDa
GenBank蛋白编号BC003552
基因名称 Calnexin
Gene ID (NCBI) 821
RRIDAB_2920054
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP27824
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

实验方案

Product Specific Protocols
WB protocol for CL594 Calnexin antibody CL594-66903Download protocol
IF protocol for CL594 Calnexin antibody CL594-66903Download protocol
Standard Protocols
Click here to view our Standard Protocols