验证数据展示
经过测试的应用
Positive IF/ICC detected in | C2C12 cells, MDCK cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-13938 targets Calponin in IF/ICC applications and shows reactivity with human, mouse, rat, dog samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse, rat, dog |
免疫原 | Calponin fusion protein Ag4989 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | calponin 1, basic, smooth muscle |
别名 | CNN1, CNN 1, Calponin-1, Calponin 1, Basic calponin |
计算分子量 | 33 kDa |
观测分子量 | 35 kDa |
GenBank蛋白编号 | BC022015 |
基因名称 | Calponin |
Gene ID (NCBI) | 1264 |
RRID | AB_2919081 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P51911 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 Calponin antibody CL488-13938 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |