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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Calponin Polyclonal antibody

Calponin Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-13938

Print datasheet

Synonyms

CNN1, CNN 1, Calponin-1, Calponin 1, Basic calponin

验证数据展示

  • IF Staining of mouse heart using CL594-13938

    IF Staining of mouse heart using CL594-13938

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite®594 Calponin antibody (CL594-13938) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of C2C12 using CL594-13938

    IF Staining of C2C12 using CL594-13938

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite®594 Calponin antibody (CL594-13938) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inC2C12 cells
For other applications, we recommend the unconjugated version of this antibody, 13938-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-13938 targets Calponin in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen Calponin fusion protein Ag4989 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calponin 1, basic, smooth muscle
Synonyms CNN1, CNN 1, Calponin-1, Calponin 1, Basic calponin
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC022015
Gene Symbol Calponin
Gene ID (NCBI) 1264
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51911
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.

实验方案

Product Specific Protocols
IF protocol for CL594 Calponin antibody CL594-13938Download protocol
Standard Protocols
Click here to view our Standard Protocols