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CoraLite® Plus 488-conjugated Centrin 1 Monoclonal antibody

Centrin 1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G8D10

Cat no : CL488-68433

Print datasheet

Synonyms

CETN1, CETN, Centrin-1, CEN1, Caltractin isoform 2

验证数据展示

  • IF Staining of hTERT-RPE1 using CL488-68433

    IF Staining of hTERT-RPE1 using CL488-68433

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 Centrin 1 antibody (CL488-68433, Clone: 1G8D10 ) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).

  • IF Staining of hTERT-RPE1 using CL488-68433

    IF Staining of hTERT-RPE1 using CL488-68433

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 Centrin 1 antibody (CL488-68433, Clone: 1G8D10 ) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).

经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells
For other applications, we recommend the unconjugated version of this antibody, 68433-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68433 targets Centrin 1 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Centrin 1 fusion protein Ag3529 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name centrin, EF-hand protein, 1
Synonyms CETN1, CETN, Centrin-1, CEN1, Caltractin isoform 2
Calculated Molecular Weight 172 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC029515
Gene Symbol Centrin 1
Gene ID (NCBI) 1068
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12798
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Centrin 1 antibody CL488-68433Download protocol
Standard Protocols
Click here to view our Standard Protocols