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验证数据展示

  • IF Staining of hTERT-RPE1 using 68433-1-Ig

    IF Staining of hTERT-RPE1 using 68433-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of ARPE-19 using 68433-1-Ig

    IF Staining of ARPE-19 using 68433-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of hTERT-RPE1 using 68433-1-Ig

    IF Staining of hTERT-RPE1 using 68433-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Centrin 1 Monoclonal antibody

Centrin 1 Monoclonal Antibody for IF/ICC, ELISA
Cat No. 68433-1-Ig

Print datasheet

CloneNo. 1G8D10

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC, ELISA

Synonyms

Caltractin isoform 2, CEN1, Centrin 1, centrin, EF hand protein, 1, CETN, CETN1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells, ARPE-19 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68433-1-Ig targets Centrin 1 in IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen Centrin 1 fusion protein Ag3529 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name centrin, EF-hand protein, 1
Synonyms Caltractin isoform 2, CEN1, Centrin 1, centrin, EF hand protein, 1, CETN, CETN1
Calculated Molecular Weight 172 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC029515
Gene Symbol Centrin 1
Gene ID (NCBI) 1068
RRIDAB_3085147
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12798
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

实验方案

Product Specific Protocols
IF protocol for Centrin 1 antibody 68433-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols