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验证数据展示

  • IF Staining of hTERT-RPE1 using 68433-1-Ig

    IF Staining of hTERT-RPE1 using 68433-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of ARPE-19 using 68433-1-Ig

    IF Staining of ARPE-19 using 68433-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of hTERT-RPE1 using 68433-1-Ig

    IF Staining of hTERT-RPE1 using 68433-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Centrin 1 Monoclonal antibody

Centrin 1 Monoclonal Antibody for IF/ICC, ELISA
Cat No. 68433-1-Ig

产品说明书

CloneNo. 1G8D10

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

IF/ICC, ELISA

别名

Caltractin isoform 2, CEN1, Centrin 1, centrin, EF hand protein, 1, CETN, CETN1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells, ARPE-19 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68433-1-Ig targets Centrin 1 in IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 Centrin 1 fusion protein Ag3529 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 centrin, EF-hand protein, 1
别名 Caltractin isoform 2, CEN1, Centrin 1, centrin, EF hand protein, 1, CETN, CETN1
计算分子量 172 aa, 20 kDa
观测分子量 20 kDa
GenBank蛋白编号BC029515
基因名称 Centrin 1
Gene ID (NCBI) 1068
RRIDAB_3085147
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ12798
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

实验方案

Product Specific Protocols
IF protocol for Centrin 1 antibody 68433-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols