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Claudin 5 Polyclonal antibody, PBS Only

Claudin 5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29767-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 29767-1-AP (same clone as 29767-1-PBS)

    WB analysis using 29767-1-AP (same clone as 29767-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29767-1-AP (Claudin 5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29767-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 29767-1-AP (same clone as 29767-1-PBS)

    IHC staining of human colon using 29767-1-AP (same clone as 29767-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29767-1-AP (Claudin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29767-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLDN5 antibody included
  • IHC staining of human colon using 29767-1-AP (same clone as 29767-1-PBS)

    IHC staining of human colon using 29767-1-AP (same clone as 29767-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29767-1-AP (Claudin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29767-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLDN5 antibody included
Planning an IHC experiment? We recommend our IHCeasy CLDN5 Ready-To-Use IHC Kit. CLDN5 primary antibody included.

产品信息

29767-1-PBS targets Claudin 5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Claudin 5 fusion protein Ag29044 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name claudin 5
Synonyms
Calculated Molecular Weight 218 aa, 23 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC032363
Gene Symbol CLDN5
Gene ID (NCBI) 7122
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00501
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 27 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.