验证数据展示
经过测试的应用
| Positive WB detected in | unboiled mouse lung tissue, unboiled rat lung tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CLDN5 Ready-To-Use IHC Kit. CLDN5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29767-1-AP targets Claudin 5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Claudin 5 fusion protein Ag29044 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | claudin 5 |
| 别名 | CLDN5, CLDN 5, Claudin-5, Claudin5, BEC1 |
| 计算分子量 | 218 aa, 23 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC032363 |
| 基因名称 | CLDN5 |
| Gene ID (NCBI) | 7122 |
| RRID | AB_2935477 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00501 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 27 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Claudin 5 antibody 29767-1-AP | Download protocol |
| IHC protocol for Claudin 5 antibody 29767-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
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|---|---|---|
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Int J Mol Sci Ursolic Acid Alleviates Neuroinflammation after Intracerebral Hemorrhage by Mediating Microglial Pyroptosis via the NF-κB/NLRP3/GSDMD Pathway | ||
Arch Biochem Biophys Serum exosomal miRNA promote glioma progression by targeting SOS1 via abscopal effect of radiation | ||
Brain Res Bull Inhibition of MMP-2 and MMP-9 attenuates surgery-induced cognitive impairment in aged mice | ||
Mol Biotechnol Omega-3 Polyunsaturated Fatty Acids Alleviate Intestinal Barrier Dysfunction in Obstructive Jaundice Rats |