Claudin 5 Polyclonal antibody

Claudin 5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 29767-1-AP

Print datasheet

Synonyms

CLDN5, CLDN 5, Claudin-5, Claudin5, BEC1



经过测试的应用

Positive WB detected inunboiled mouse lung tissue, unboiled rat lung tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CLDN5 Ready-To-Use IHC Kit. CLDN5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29767-1-AP targets Claudin 5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Claudin 5 fusion protein Ag29044 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name claudin 5
Synonyms CLDN5, CLDN 5, Claudin-5, Claudin5, BEC1
Calculated Molecular Weight 218 aa, 23 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC032363
Gene Symbol CLDN5
Gene ID (NCBI) 7122
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00501
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 27 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

实验方案

Product Specific Protocols
WB protocol for Claudin 5 antibody 29767-1-APDownload protocol
IHC protocol for Claudin 5 antibody 29767-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Life Sci

Metabolomics study reveals increased deoxycholic acid contributes to deoxynivalenol-mediated intestinal barrier injury

Authors - Xin He
ratWB

Int J Mol Sci

Ursolic Acid Alleviates Neuroinflammation after Intracerebral Hemorrhage by Mediating Microglial Pyroptosis via the NF-κB/NLRP3/GSDMD Pathway

Authors - Pan Lei
mouseWB

Brain Res Bull

Inhibition of MMP-2 and MMP-9 attenuates surgery-induced cognitive impairment in aged mice

Authors - Yiqin Ji
ratWB

Mol Biotechnol

Omega-3 Polyunsaturated Fatty Acids Alleviate Intestinal Barrier Dysfunction in Obstructive Jaundice Rats

Authors - Changxi Zhang
mouseIF

Nan Fang Yi Ke Da Xue Xue Bao

[Aqueous extract of Chuan Xiong Rhizoma enhances inhibitory effect of temozolomide against brain metastasis of melanoma in mice]

Authors - Q Zhao
humanWB

iScience

A single cell atlas of circulating immune cells involved in diabetic retinopathy

Authors - Dan Liao