Collagen Type I Monoclonal antibody
Collagen Type I Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig and More (2)
Applications
WB, IHC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
172
CloneNo.
1E9A7
验证数据展示
经过测试的应用
Positive WB detected in | pig colon tissue, human cervical cancer tissue, human placenta tissue, pig lung tissue, pig skin tissue |
Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human colon cancer tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67288-1-Ig targets Collagen Type I in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, pig |
Cited Reactivity | human, pig, rabbit, zebrafish |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | collagen, type I, alpha 1 |
Synonyms | COL1A1, col1, COL I, Alpha-1 type I collagen, Alpha 1 type I collagen |
Calculated Molecular Weight | 139 kDa |
Observed Molecular Weight | 120-130 kDa |
GenBank Accession Number | NM_000088 |
Gene Symbol | COL1A1 |
Gene ID (NCBI) | 1277 |
RRID | AB_2882554 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P02452 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Collagen Type I antibody 67288-1-Ig | Download protocol |
IHC protocol for Collagen Type I antibody 67288-1-Ig | Download protocol |
IF protocol for Collagen Type I antibody 67288-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Adv Mater Biodegradable Piezoelectric-Conductive Integrated Hydrogel Scaffold for Repair of Osteochondral Defects | ||
J Hematol Oncol Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma | ||
Nat Cell Biol Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis. | ||
Adv Sci (Weinh) Silencing of NOTCH3 Signaling in Meniscus Smooth Muscle Cells Inhibits Fibrosis and Exacerbates Degeneration in a HEYL-Dependent Manner | ||
Small M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation | ||
Eur Respir J Intracellular hydroxyproline imprinting following resolution of bleomycin-induced pulmonary fibrosis. |