Collagen Type I Monoclonal antibody

Collagen Type I Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig and More (2)

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1E9A7

Cat no : 67288-1-Ig

Print datasheet

Synonyms

COL1A1, col1, COL I, Alpha-1 type I collagen, Alpha 1 type I collagen



经过测试的应用

Positive WB detected inpig colon tissue, human cervical cancer tissue, human placenta tissue, pig lung tissue, pig skin tissue
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy COL1A1 Ready-To-Use IHC Kit. COL1A1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67288-1-Ig targets Collagen Type I in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, pig
Cited Reactivityhuman, pig, rabbit, zebrafish
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name collagen, type I, alpha 1
Synonyms COL1A1, col1, COL I, Alpha-1 type I collagen, Alpha 1 type I collagen
Calculated Molecular Weight139 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberNM_000088
Gene Symbol COL1A1
Gene ID (NCBI) 1277
RRIDAB_2882554
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02452
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727).

实验方案

Product Specific Protocols
WB protocol for Collagen Type I antibody 67288-1-IgDownload protocol
IHC protocol for Collagen Type I antibody 67288-1-IgDownload protocol
IF protocol for Collagen Type I antibody 67288-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigIF

Adv Mater

Biodegradable Piezoelectric-Conductive Integrated Hydrogel Scaffold for Repair of Osteochondral Defects

Authors - Dingge Liu
humanIHC

J Hematol Oncol

Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma

Authors - Yexin Tong
humanIF

Nat Cell Biol

Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.

Authors - Yoshihiko Kobayashi
humanIHC

Adv Sci (Weinh)

Silencing of NOTCH3 Signaling in Meniscus Smooth Muscle Cells Inhibits Fibrosis and Exacerbates Degeneration in a HEYL-Dependent Manner

Authors - Hao Sun
WB,IHC

Small

M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation

Authors - Jie Sun
WB

Eur Respir J

Intracellular hydroxyproline imprinting following resolution of bleomycin-induced pulmonary fibrosis.

Authors - Shengren Song