CoraLite®594-conjugated Collagen Type I Monoclonal antibody

Collagen Type I Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse and More (1)

Applications

IF-P and More (1)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E9A7

Cat no : CL594-67288

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Synonyms

Alpha 1 type I collagen, COL1A1, Collagen alpha 1(I) chain, Collagen Type I, collagen, type I, alpha 1, OI4



经过测试的应用

Positive IF-P detected inhuman colon cancer tissue, human breast cancer tissue, mouse skin tissue
Planning an IHC experiment? We recommend our IHCeasy COL1A1 Ready-To-Use IHC Kit. COL1A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67288-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL594-67288 targets Collagen Type I in IF-P applications and shows reactivity with Human, Mouse samples.

Tested Applications IF-P Application Description
Cited ApplicationsIF
Tested Reactivity Human, Mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name collagen, type I, alpha 1
Synonyms Alpha 1 type I collagen, COL1A1, Collagen alpha 1(I) chain, Collagen Type I, collagen, type I, alpha 1, OI4
Calculated Molecular Weight139 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberNM_000088
Gene Symbol COL1A1
Gene ID (NCBI) 1277
RRIDAB_2920107
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02452
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain.

实验方案

Product Specific Protocols
IF protocol for CL594 Collagen Type I antibody CL594-67288Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Int J Biol Sci

Bulk and single-cell transcriptome profiling reveal extracellular matrix mechanical regulation of lipid metabolism reprograming through YAP/TEAD4/ACADL axis in hepatocellular carcinoma

Authors - Jingwei Cai