CoraLite® Plus 488-conjugated Collagen Type I Monoclonal antibody
Collagen Type I Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig
Applications
IF-P and More (1)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1E9A7
验证数据展示
经过测试的应用
| Positive IF-P detected in | human colon cancer tissue, mouse skin tissue |
Planning an IHC experiment? We recommend our IHCeasy COL1A1 Ready-To-Use IHC Kit. COL1A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67288-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-67288 targets Collagen Type I in IF-P applications and shows reactivity with human, mouse, pig samples.
| Tested Applications | IF-P Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | collagen, type I, alpha 1 |
| Synonyms | COL1A1, col1, COL I, Alpha-1 type I collagen, Alpha 1 type I collagen |
| Calculated Molecular Weight | 139 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | NM_000088 |
| Gene Symbol | COL1A1 |
| Gene ID (NCBI) | 1277 |
| RRID | AB_2919445 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P02452 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Collagen Type I antibody CL488-67288 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Med Virol Molecular and immune signatures, and pathological trajectories of fatal COVID-19 lungs defined by in situ spatial single-cell transcriptome analysis |