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验证数据展示

  • IF Staining of mouse skeletal muscle using CL488-17023

    IF Staining of mouse skeletal muscle using CL488-17023

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite® Plus 488 Collagen Type VI antibody (CL488-17023) at dilution of 1:200.

  • IF Staining of human colon using CL488-17023

    IF Staining of human colon using CL488-17023

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using CoraLite® Plus 488 Collagen Type VI antibody (CL488-17023) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated COL6A1 Polyclonal antibody

COL6A1 Polyclonal Antibody for IF-P
Cat No. CL488-17023

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IF-P

别名

COL6, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain, Collagen Type VI

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse skeletal muscle tissue, human colon tissue
Planning an IHC experiment? We recommend our IHCeasy COL6A1 Ready-To-Use IHC Kit. COL6A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17023-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17023 targets COL6A1 in IF-P applications and shows reactivity with mouse samples.

经测试应用 IF-P Application Description
经测试反应性 mouse
免疫原 COL6A1 fusion protein Ag10288 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen, type VI, alpha 1
别名 COL6, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain, Collagen Type VI
计算分子量 1028 aa, 109 kDa
观测分子量 140 kDa
GenBank蛋白编号BC052575
基因名称 COL6A1
Gene ID (NCBI) 1291
RRIDAB_3672666
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12109
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 COL6A1 antibody CL488-17023Download protocol
Standard Protocols
Click here to view our Standard Protocols