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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Collagen Type VI Polyclonal antibody

Collagen Type VI Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17023

Print datasheet

Synonyms

COL6A1, COL6, OPLL, Collagen alpha-1(VI) chain, Collagen alpha 1(VI) chain

验证数据展示

  • IF Staining of mouse skeletal muscle using CL488-17023

    IF Staining of mouse skeletal muscle using CL488-17023

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite® Plus 488 Collagen Type VI antibody (CL488-17023) at dilution of 1:200.

  • IF Staining of human colon using CL488-17023

    IF Staining of human colon using CL488-17023

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using CoraLite® Plus 488 Collagen Type VI antibody (CL488-17023) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF-P detected inmouse skeletal muscle tissue, human colon tissue
Planning an IHC experiment? We recommend our IHCeasy COL6A1 Ready-To-Use IHC Kit. COL6A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17023-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17023 targets Collagen Type VI in IF-P applications and shows reactivity with mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity mouse
Immunogen Collagen Type VI fusion protein Ag10288 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen, type VI, alpha 1
Synonyms COL6A1, COL6, OPLL, Collagen alpha-1(VI) chain, Collagen alpha 1(VI) chain
Calculated Molecular Weight 1028 aa, 109 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC052575
Gene Symbol COL6A1
Gene ID (NCBI) 1291
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12109
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Collagen Type VI antibody CL488-17023Download protocol
Standard Protocols
Click here to view our Standard Protocols