• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

Collagen Type VI Polyclonal antibody

Collagen Type VI Polyclonal Antibody for WB, IHC, IF, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 17023-1-AP

Print datasheet

Synonyms

COL6A1, COL6, OPLL, Collagen alpha-1(VI) chain, Collagen alpha 1(VI) chain

验证数据展示

  • IF Staining of mouse skeletal muscle tissue using 17023-1-AP

    IF Staining of mouse skeletal muscle tissue using 17023-1-AP

    IF result of anti-COL6A1 (17023-1-AP, 1:500) with PFA fixed mouse skeletal muscle tissue by Dr. Daniel Kopinke.

  • IHC staining of human colon using 17023-1-AP

    IHC staining of human colon using 17023-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL6A1 antibody included
  • IHC staining of human colon using 17023-1-AP

    IHC staining of human colon using 17023-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL6A1 antibody included
  • WB analysis using 17023-1-AP

    WB analysis using 17023-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17023-1-AP (Collagen Type VI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293T using 17023-1-AP

    WB analysis of HEK-293T using 17023-1-AP

    WB result of Collagen Type VI antibody (17023-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Collagen Type VI transfected HEK-293T cells.

  • IP experiment of mouse heart using 17023-1-AP

    IP experiment of mouse heart using 17023-1-AP

    IP result of anti-Collagen Type VI (IP:17023-1-AP, 4ug; Detection:17023-1-AP 1:2000) with mouse heart tissue lysate 1050 ug.

  • IHC staining of human colon using 17023-1-AP

    IHC staining of human colon using 17023-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COL6A1 antibody included
  • IF Staining of human colon using 17023-1-AP

    IF Staining of human colon using 17023-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human colon using 17023-1-AP

    IF Staining of human colon using 17023-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse skeletal muscle using 17023-1-AP

    IF Staining of mouse skeletal muscle using 17023-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse heart tissue, HEK-293T cells, human heart tissue, human skeletal muscle tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman colon tissue, human liver tissue, mouse liver tissue, human heart tissue, human hepatocirrhosis tissue, human kidney tissue, human placenta tissue, human testis tissue, human spleen tissue, human lung tissue, human ovary tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon tissue, mouse skeletal muscle tissue
Positive IF detected inmouse skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy COL6A1 Ready-To-Use IHC Kit. COL6A1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17023-1-AP targets Collagen Type VI in WB, IHC, IF, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, zebrafish, sheep
Immunogen Collagen Type VI fusion protein Ag10288 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen, type VI, alpha 1
Synonyms COL6A1, COL6, OPLL, Collagen alpha-1(VI) chain, Collagen alpha 1(VI) chain
Calculated Molecular Weight 1028 aa, 109 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC052575
Gene Symbol COL6A1
Gene ID (NCBI) 1291
RRIDAB_2229737
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12109
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

实验方案

Product Specific Protocols
WB protocol for Collagen Type VI antibody 17023-1-APDownload protocol
IHC protocol for Collagen Type VI antibody 17023-1-APDownload protocol
IF protocol for Collagen Type VI antibody 17023-1-APDownload protocol
IP protocol for Collagen Type VI antibody 17023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,ratIF

Cell Death Differ

Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation

Authors - Guangjin Chen
View Article
zebrafishWB,IF

Cancer Res

Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis

Authors - Ying Liu
View Article
human,mouseWB,IF

Cell Rep

The mechano-chemical circuit in fibroblasts and dendritic cells drives basal cell proliferation in psoriasis

Authors - Jingwei Jiang
View Article
humanIF

Matrix Biol

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Authors - Emna Ouni
View Article
humanWB,IHC

Oncogene

Twist1-induced activation of human fibroblasts promotes matrix stiffness by upregulating palladin and collagen α1(VI).

Authors - I García-Palmero
View Article
humanWB,IHC

Cancer Gene Ther

Schwann cell derived pleiotrophin stimulates fibroblast for proliferation and excessive collagen deposition in plexiform neurofibroma

Authors - Zhuowei Tian
View Article