CoraLite® Plus 488-conjugated Complement factor B Monoclonal antibody
Complement factor B Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgM
Reactivity
human
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
8D2B6
验证数据展示
经过测试的应用
| Positive IF-P detected in | human liver tissue |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
For other applications, we recommend the unconjugated version of this antibody, 66154-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66154 targets Complement factor B in IF-P applications and shows reactivity with human samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | human |
| Immunogen | Complement factor B fusion protein Ag19107 种属同源性预测 |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | complement factor B |
| Synonyms | BF, BFD, C3/C5 convertase, CFAB, CFB, complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B |
| Calculated Molecular Weight | 86 kDa |
| Observed Molecular Weight | 93 kDa |
| GenBank Accession Number | BC007990 |
| Gene Symbol | Complement factor B |
| Gene ID (NCBI) | 629 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Thiophilic affinity chromatograph |
| UNIPROT ID | P00751 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is cleaved into Ba and Bb by factor D in the presence of C3b. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Complement factor B antibody CL488-66154 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |