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CoraLite®594-conjugated Complement factor B Monoclonal antibody

Complement factor B Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

8D2B6

Cat no : CL594-66154

Print datasheet

Synonyms

BF, BFD, C3/C5 convertase, CFAB, CFB, complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B

验证数据展示

  • IF Staining of human liver using CL594-66154

    IF Staining of human liver using CL594-66154

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite®594 Complement factor B antibody (CL594-66154, Clone: 8D2B6 ) at dilution of 1:200.

  • IF Staining of human liver using CL594-66154

    IF Staining of human liver using CL594-66154

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite®594 Complement factor B antibody (CL594-66154, Clone: 8D2B6 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman liver tissue

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

For other applications, we recommend the unconjugated version of this antibody, 66154-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66154 targets Complement factor B in IF-P applications and shows reactivity with human samples.

Tested Applications IF-P Application Description
Tested Reactivity human
Immunogen Complement factor B fusion protein Ag19107 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name complement factor B
Synonyms BF, BFD, C3/C5 convertase, CFAB, CFB, complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 93 kDa
GenBank Accession NumberBC007990
Gene Symbol Complement factor B
Gene ID (NCBI) 629
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP00751
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is cleaved into Ba and Bb by factor D in the presence of C3b. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.

实验方案

Product Specific Protocols
IF protocol for CL594 Complement factor B antibody CL594-66154Download protocol
Standard Protocols
Click here to view our Standard Protocols