验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse heart tissue |
Planning an IHC experiment? We recommend our IHCeasy GJA1/Connexin 43 Ready-To-Use IHC Kit. GJA1/Connexin 43 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80543-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-80543 targets Connexin 43 in IF-P applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | Human, Mouse, Rat, Pig |
| 免疫原 | Connexin 43 fusion protein Ag25533 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | gap junction protein, alpha 1, 43kDa |
| 别名 | Connexin 43, CX43, DFNB38, Gap junction alpha 1 protein, GJA1, GJAL, ODDD |
| 计算分子量 | 43 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC026329 |
| 基因名称 | Connexin-43 |
| Gene ID (NCBI) | 2697 |
| RRID | AB_3673036 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P17302 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Connexin 43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Connexin 43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Connexin 43 antibody CL488-80543 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |