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验证数据展示

  • FC experiment of MCF-7 using CL488-10823

    FC experiment of MCF-7 using CL488-10823

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Cystatin B Polyclonal Antibody (CL488-10823)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A431 using CL488-10823

    IF Staining of A431 using CL488-10823

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 Cystatin B antibody (CL488-10823) at dilution of 1:200, CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cystatin B Polyclonal antibody

Cystatin B Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10823

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

CSTB, EPM1, Cystatin-B, CST6, CPI-B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CSTB Ready-To-Use IHC Kit. CSTB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10823-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10823 targets Cystatin B in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen Cystatin B fusion protein Ag1262 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cystatin B (stefin B)
Synonyms CSTB, EPM1, Cystatin-B, CST6, CPI-B
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC003370
Gene Symbol Cystatin B
Gene ID (NCBI) 1476
RRIDAB_3672467
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen Affinity Purified
UNIPROT IDP04080
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cystatin B (CSTB), a member of the cystatin superfamily protein, is a stefin that functions as an intracellular thiol protease inhibitor and has been thought to play a role in protecting against the proteases leaking from lysosomes. CSTB plays various functions in a variety of diseases, including epithelial ovarian cancer, colon cancer, and myoclonus epilepsy. Evidence indicates that mutations in CSTB are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cystatin B antibody CL488-10823Download protocol
Standard Protocols
Click here to view our Standard Protocols