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验证数据展示

  • FC experiment of MCF-7 using CL488-10823

    FC experiment of MCF-7 using CL488-10823

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Cystatin B Polyclonal Antibody (CL488-10823)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A431 using CL488-10823

    IF Staining of A431 using CL488-10823

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 Cystatin B antibody (CL488-10823) at dilution of 1:200, CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Cystatin B Polyclonal antibody

Cystatin B Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10823

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

CSTB, EPM1, Cystatin-B, CST6, CPI-B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CSTB Ready-To-Use IHC Kit. CSTB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10823-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10823 targets Cystatin B in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 Cystatin B fusion protein Ag1262 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cystatin B (stefin B)
别名 CSTB, EPM1, Cystatin-B, CST6, CPI-B
计算分子量 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC003370
基因名称 Cystatin B
Gene ID (NCBI) 1476
RRIDAB_3672467
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen Affinity Purified
UNIPROT IDP04080
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cystatin B (CSTB), a member of the cystatin superfamily protein, is a stefin that functions as an intracellular thiol protease inhibitor and has been thought to play a role in protecting against the proteases leaking from lysosomes. CSTB plays various functions in a variety of diseases, including epithelial ovarian cancer, colon cancer, and myoclonus epilepsy. Evidence indicates that mutations in CSTB are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cystatin B antibody CL488-10823Download protocol
Standard Protocols
Click here to view our Standard Protocols