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验证数据展示

  • IF Staining of A431 using CL594-83276

    IF Staining of A431 using CL594-83276

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 Cytochrome c antibody (CL594-83276, Clone: 230503E10 ) at dilution of 1:200.

  • IF Staining of MCF-7 using CL594-83276

    IF Staining of MCF-7 using CL594-83276

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 Cytochrome c antibody (CL594-83276, Clone: 230503E10 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated Cytochrome c Recombinant antibody

Cytochrome c Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL594-83276

产品说明书

CloneNo. 230503E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

CYCS, CYC, 230503E10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells, MCF-7
Planning an IHC experiment? We recommend our IHCeasy CYCS Ready-To-Use IHC Kit. CYCS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83276-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-83276 targets Cytochrome c in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 Cytochrome c fusion protein Ag1455 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome c, somatic
别名 CYCS, CYC, 230503E10
计算分子量 12 kDa
观测分子量 12-15 kDa
GenBank蛋白编号BC009578
基因名称 Cytochrome c
Gene ID (NCBI) 54205
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP99999
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.

实验方案

Product Specific Protocols
IF protocol for CL594 Cytochrome c antibody CL594-83276Download protocol
Standard Protocols
Click here to view our Standard Protocols