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验证数据展示

  • IF Staining of A431 using CL594-83276

    IF Staining of A431 using CL594-83276

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 Cytochrome c antibody (CL594-83276, Clone: 230503E10 ) at dilution of 1:200.

  • IF Staining of MCF-7 using CL594-83276

    IF Staining of MCF-7 using CL594-83276

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 Cytochrome c antibody (CL594-83276, Clone: 230503E10 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Cytochrome c Recombinant antibody

Cytochrome c Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL594-83276

Print datasheet

CloneNo. 230503E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

CYCS, CYC, 230503E10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells, MCF-7
Planning an IHC experiment? We recommend our IHCeasy CYCS Ready-To-Use IHC Kit. CYCS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83276-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-83276 targets Cytochrome c in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen Cytochrome c fusion protein Ag1455 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytochrome c, somatic
Synonyms CYCS, CYC, 230503E10
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 12-15 kDa
GenBank Accession NumberBC009578
Gene Symbol Cytochrome c
Gene ID (NCBI) 54205
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP99999
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.

实验方案

Product Specific Protocols
IF protocol for CL594 Cytochrome c antibody CL594-83276Download protocol
Standard Protocols
Click here to view our Standard Protocols