验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL750-83276 targets Cytochrome c in WB, FC (Intra) applications and shows reactivity with human, mouse samples.
经测试应用 | WB, FC (Intra) Application Description |
经测试反应性 | human, mouse |
免疫原 | Cytochrome c fusion protein Ag1455 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | cytochrome c, somatic |
别名 | CYCS, CYC, 230503E10 |
计算分子量 | 12 kDa |
观测分子量 | 12-15 kDa |
GenBank蛋白编号 | BC009578 |
基因名称 | Cytochrome c |
Gene ID (NCBI) | 54205 |
RRID | AB_3673778 |
偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
最大激发/发射波长 | 755 nm / 780 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P99999 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.
实验方案
Product Specific Protocols | |
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WB protocol for CL Plus 750 Cytochrome c antibody CL750-83276 | Download protocol |
FC protocol for CL Plus 750 Cytochrome c antibody CL750-83276 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |