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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated Cytochrome c Recombinant antibody

Cytochrome c Uni-rAbTM Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230503E10

Cat no : CL750-83276

Print datasheet

Synonyms

CYCS, CYC, 230503E10

验证数据展示

  • FC experiment of HepG2 using CL750-83276

    FC experiment of HepG2 using CL750-83276

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 750 Cytochrome C Recombinant Antibody (CL750-83276, Clone:230503E10)(red), or 0.8 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using CL750-83276

    WB analysis using CL750-83276

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-83276 (Cytochrome c antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CYCS Ready-To-Use IHC Kit. CYCS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83276-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL750-83276 is Cytochrome c Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen Cytochrome c fusion protein Ag1455 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytochrome c, somatic
Synonyms CYCS, CYC, 230503E10
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 12-15 kDa
GenBank Accession NumberBC009578
Gene Symbol Cytochrome c
Gene ID (NCBI) 54205
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP99999
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. As a part of respiratory chain, cytochrome c plays a critical role in the process of oxidative phosphorylation and ATP producing. Besides, cytochrome c also gets implicated in apoptosis process. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, which is required for caspase-3 activation and the occurrence of apoptosis.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 Cytochrome c antibody CL750-83276Download protocol
FC protocol for CL Plus 750 Cytochrome c antibody CL750-83276Download protocol
Standard Protocols
Click here to view our Standard Protocols